Significantly different types of mineralization in the root canals of groups 2 and 3 were seen ( P = 0.001). Atubular tertiary dentine deposition in the form of matrix deposition along root canal walls, or dentine bridge formation at the orifice of root canals or complete pulp canal obliteration, was found after treatment of dentine with EDTA in both experiments (groups 2 and 3). Teeth after treatment of dentine with saline (group 1) were associated with no or only traces of hard tissue formation along the root canal walls. Data were compared using the nonparametric Fisher's exact test. Teeth were evaluated histo-morphologically after 10 weeks. The access cavities were restored with a Teflon disc and glass ionomer. The film was removed, and the pulp chamber cavities were left empty (groups 1 and 2), or filled with swine collagenous sponge (group 3). The circumpulpal pulp chamber dentine was treated for 3 min with normal saline (group 1), or 17% EDTA solution (groups 2 and 3). ![]() The haemorrhage was controlled, and the root pulp was protected using a polyurethane film. After preparation of pulp exposures through Class I cavities, the tissue of the pulp chamber was completely removed. Methodologyįorty-two fully developed permanent premolars and molars of healthy miniature swines were used. To investigate pulp responses after pulpotomy and EDTA conditioning of pulp chamber dentinal walls with or without the placement of a collagenous scaffold in the experimental model of miniature swine teeth.
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